The Center for Advanced Imaging at Harvard is located in the Northwest Building (52 Oxford Street, Cambridge, MA 02138), a multi-disciplinary research building designed to enhance collaboration and housing neuroscience, bioengineering, computational analysis and other programs under one roof. It has become a model for a new generation of research environments.

The Center occupies approximately 5200 square feet on two floors including a state of the art imaging laboratory (3654 square feet on the B4 floor) and office suite (1511 square feet on the ground floor). Space in the Center's laboratory is a combination of open laboratory space, optics rooms and support spaces such as equipment and environmentally controlled room.